APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
Protein-Typ
Recombinant
Proteineigenschaft
AA 20-345
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses APOH Protein ist gelabelt mit His tag.
Verwendungszweck
Human APOH Protein
Sequenz
Gly20-Cys345
Produktmerkmale
Recombinant Human APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human APOH, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.28μg/ml determined by ELISA. See testing image for detail.
APOH
Spezies: Human
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
APOH
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
Molekulargewicht
37.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.