Dieses AXL Protein ist gelabelt mit Fc Tag,Biotin.
Verwendungszweck
Biotinylated Human Axl Protein (Primary Amine Labeling)
Sequenz
Ala26-Pro449
Produktmerkmale
Recombinant Biotinylated Human Axl Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala26-Pro449.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-Axl Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA. See testing image for detail.
AXL
Spezies: Maus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Hintergrund
Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Molekulargewicht
72.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.