Recombinant Human BACE-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr22-Thr457, which will be further processed into Thr22-Thr457(pro-form) & Glu46-Thr457(mature-form).
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
BACE1
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The beta-site amyloid precursor protein cleaving enzyme-1 (BACE-1) initiates the generation of amyloid-β (Aβ), and the amyloid cascade leading to amyloid plaque deposition, neurodegeneration, and dementia in Alzheimer's disease (AD). Clinical failures of anti-Aβ therapies in dementia stages suggest that treatment has to start in the early, asymptomatic disease states.
Molekulargewicht
75.2 kDa (pro-form) and 72.6 kDa (mature-form). Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.