BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
Protein-Typ
Recombinant
Proteineigenschaft
Trimer
Spezies
Cynomolgus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses BAFF Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein
Sequenz
Thr141-Leu285
Produktmerkmale
Recombinant Cynomolgus BAFF / TNFSF13B / CD257 Trimer Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Thr141-Leu285.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus BAFF Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.53μg/ml determined by ELISA. See testing image for detail.
TNFSF13B
Spezies: Human
Wirt: Tobacco (Nicotiana benthamiana)
Recombinant
> 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
WB, CC
Active
TNFSF13B
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
BAFF (TNFSF13B)
(Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Molekulargewicht
54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.