VTCN1
(V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
Protein-Typ
Recombinant
Proteineigenschaft
AA 29-258
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses VTCN1 Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human B7-H4 Protein
Sequenz
Phe29-Ala258
Produktmerkmale
Recombinant Human B7-H4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Ala258.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human B7-H4, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H4 Antibody, hFc Tag with the EC50 of 7ng/ml determined by ELISA. See testing image for detail.
VTCN1
Spezies: Maus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
VTCN1
(V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))
B7h4 Protein, B7s1 Protein, B7x Protein, BC032925 Protein, B7-H4 Protein, B7H4 Protein, B7S1 Protein, B7X Protein, B7h.5 Protein, PRO1291 Protein, VCTN1 Protein, RGD1311204 Protein, V set domain containing T cell activation inhibitor 1 L homeolog Protein, V-set domain containing T cell activation inhibitor 1 Protein, V-set domain containing T-cell activation inhibitor 1 Protein, V set domain containing T cell activation inhibitor 1 Protein, vtcn1.L Protein, VTCN1 Protein, vtcn1 Protein, Vtcn1 Protein
Hintergrund
B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .
Molekulargewicht
52.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.