Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln24-Lys265.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human EpCAM, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
EPCAM
Spezies: Ratte
Wirt: HEK-293 Cells
Recombinant
> 97 % as determined by SDS-PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF-like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Molekulargewicht
28.2 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.