Dieses Poliovirus Receptor Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human CD155/PVR Protein
Sequenz
Trp21-Asn343
Produktmerkmale
Recombinant Human CD155/PVR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp21-Asn343.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by SEC-HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human CD155, hFc Tag with the EC50 of 0.18μg/ml determined by ELISA. The affinity constant of 0.958 μM as determined in SPR assay (Biacore T200). See testing image for detail.
PVR
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
Molekulargewicht
61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.