> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD200 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 18ng/ml determined by ELISA. See testing image for detail.
CD200
Spezies: Maus
Wirt: CHO Cells
Recombinant
>98 % (SDS-PAGE)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD200, MOX1, MOX2, My033, MRC, OX-2, OX2,CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.
Molekulargewicht
23.5 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.