Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD3D & CD3E protein (His tag)

Spezies: Human Wirt: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Produktnummer ABIN7274136
  • Target
    CD3D & CD3E
    Protein-Typ
    Recombinant
    Spezies
    • 9
    • 7
    • 4
    • 2
    • 2
    Human
    Quelle
    • 22
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    His tag
    Sequenz
    Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)
    Reinheit
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    CD3D & CD3E
    Andere Bezeichnung
    CD3E&CD3D
    Synonyme
    AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
    Hintergrund
    CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon,T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
    Molekulargewicht
    17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 35-42 kDa and 25 kDa based on Tris-Bis PAGE result.
Sie sind hier:
Kundenservice