Dieses CD5 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Rhesus macaque CD5 Protein
Sequenz
Arg25-Pro375
Produktmerkmale
Recombinant Biotinylated Rhesus macaque CD5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Pro375.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Rhesus macaque CD5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA. See testing image for detail.
CD5
Spezies: Ratte
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion, expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Molekulargewicht
41.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.