FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 36-218
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses FCGR2A Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Human Fc gamma RIIA/CD32a (H167) Protein
Sequenz
Ala36-Ile218 (H167)
Produktmerkmale
Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(H167).
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
The affinity constant of 0.606 μM as determined in SPR assay (Biacore T200). See testing image for detail.
FCGR2A
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
FCGR2A
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
CD32 Protein, CD32A Protein, CDw32 Protein, FCG2 Protein, FCGR2 Protein, FCGR2A1 Protein, FcGR Protein, IGFR2 Protein, CD32a Protein, Fc fragment of IgG receptor IIa Protein, FCGR2A Protein
Hintergrund
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains, these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Molekulargewicht
23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.