Dieses LI Cadherin Protein ist gelabelt mit His tag.
Verwendungszweck
Human CDH17/Cadherin 17 Domain 1-6 Protein
Sequenz
Gln23-Leu667
Produktmerkmale
Recombinant Human CDH17/Cadherin 17 Domain 1-6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Leu667.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CDH17 Domain 1-6, His Tag at 1_g/ml (100_l/Well) on the plate. Dose response curve for Anti-CDH17 Anitibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl ( pH 8.0).. Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades.In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.
Molekulargewicht
72.90 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.