Dieses LI Cadherin Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human CDH17/Cadherin 17 Protein
Sequenz
Gln23-Met787
Produktmerkmale
Recombinant Biotinylated Human CDH17/Cadherin 17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln23-Met787.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CDH17 Antibody at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CDH17, His Tag with the EC50 of 26.1ng/ml determined by ELISA. See testing image for detail.
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Liver-intestine cadherin (CDH17) has been known to function as a tumor stimulator and diagnostic marker for almost two decades.In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.
Molekulargewicht
87.88 kDa. Due to glycosylation, the protein migrates to 105-120 kDa based on Tris-Bis PAGE result.