Dieses CTGF Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human CTGF/CCN2 Protein
Sequenz
Gln27-Ala349
Produktmerkmale
Recombinant Biotinylated Human CTGF/CCN2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln27-Ala349.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CTGF Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 32.6ng/ml determined by ELISA. See testing image for detail.
CTGF
Spezies: Rhesusaffen
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
Molekulargewicht
38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result.