CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
Protein-Typ
Recombinant
Proteineigenschaft
AA 65-265
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses CLEC12A Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human CLEC12A/MICL/CLL-1 Protein
Sequenz
His65-Ala265
Produktmerkmale
Recombinant Biotinylated Human CLEC12A/MICL/CLL-1 Protein is expressed from HEK293 with His tag at the N-Terminus and Avi tag at the C-Terminus.It contains His65-Ala265.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CLEC12A Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CLEC12A, His Tag with the EC50 of 0.41μg/ml determined by ELISA. See testing image for detail.
CLEC12A
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
CLEC12A
(C-Type Lectin Domain Family 12, Member A (CLEC12A))
MGC179455 Protein, CLL-1 Protein, CLL1 Protein, DCAL-2 Protein, MICL Protein, D230024O04 Protein, KLRL1 Protein, Micl Protein, C-type lectin domain family 12 member A Protein, C-type lectin domain family 12, member a Protein, C-type lectin domain family 12, member A Protein, CLEC12A Protein, Clec12a Protein
Hintergrund
CLEC12A has recently been identified as an antigen, expressed on leukemic stem cells and leukemic blasts. Given the fact that this expression profile seems stable throughout diagnosis, treatment and relapse on leukemic blasts and leukemic stem cells, CLEC12A can be considered a highly potent and reliable marker for the detection of measurable residual disease and therefore applicable for risk stratification and prognostication in AML.
Molekulargewicht
26.4 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.