SARS-CoV-2 Spike Protein (RBD) (His-Avi Tag)
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- Target Alle SARS-CoV-2 Spike Proteine anzeigen
- SARS-CoV-2 Spike
- Protein-Typ
- Recombinant
- Proteineigenschaft
- RBD
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Spezies
- SARS Coronavirus-2 (SARS-CoV-2)
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses SARS-CoV-2 Spike Protein ist gelabelt mit His-Avi Tag.
- Sequenz
- Arg319-Asn532
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma Wirt: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (B.1.617.2 - delta), (RBD) protein (Fc Tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Delta Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (B.1.1.7 - alpha), (RBD) protein (Fc Tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (D614G), (Trimer) protein (His-Avi Tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (BA.2 - Omicron), (RBD) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Produkte
- Synonyme
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Hintergrund
- S protein RBD, S glycoprotein RBD, Spike protein RBD,The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molekulargewicht
- 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
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