CRTAM
(Cytotoxic and Regulatory T Cell Molecule (CRTAM))
Protein-Typ
Recombinant
Proteineigenschaft
AA 18-287
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses CRTAM Protein ist gelabelt mit His tag.
Verwendungszweck
Human CRTAM Protein
Sequenz
Ser18-Gly287
Produktmerkmale
Recombinant Human CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser18-Gly287.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CRTAM, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 57.3ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
CRTAM
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
CRTAM
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
WB
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
CRTAM
(Cytotoxic and Regulatory T Cell Molecule (CRTAM))
TAD Protein, cTADS Protein, MGC82176 Protein, CRTAM Protein, CD355 Protein, cytotoxic and regulatory T-cell molecule Protein, cytotoxic and regulatory T-cell molecule L homeolog Protein, cytotoxic and regulatory T cell molecule Protein, CRTAM Protein, crtam.L Protein, Crtam Protein
Hintergrund
Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile, therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.
Molekulargewicht
31.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.