Dieses PF4 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human CXCL4 Protein
Sequenz
Glu32-Ser101
Produktmerkmale
Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-CXCL4 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 2.3μg/ml determined by ELISA. See testing image for detail.
PF4
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
PF4
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by SDS-PAGE
Func
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 4 mM HCl ( pH 3.0).
Buffer
Lyophilized from 0.22μm filtered solution in 4 mM HCl ( pH 3.0). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.
Molekulargewicht
10.68 kDa. The protein migrates to 13 kDa based on Tris-Bis PAGE result.