Recombinant Cynomolgus DLL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Ala477.
Reinheit
> 90 % as determined by Tris-Bis PAGE,> 90 % as determined by SEC-HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus DLL3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
DLL3
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 80 % as determined by SDS-PAGE and Coomassie blue staining.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM Tris, 200 mM NaCl, 200 mM L-arginine ( pH 8.5).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SCDO1 Protein, pu Protein, pudgy Protein, delta like canonical Notch ligand 3 Protein, delta-like 3 (Drosophila) Protein, DLL3 Protein, Dll3 Protein
Hintergrund
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Molekulargewicht
48.80 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.