Dieses DLL3 Protein ist gelabelt mit His-DYKDDDDK Tag.
Verwendungszweck
Human DLL3 (27-215) Protein
Sequenz
Ala27-Glu215
Produktmerkmale
Recombinant Human DLL3 (27-215) Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala27-Glu215.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human DLL3 (27-215) , His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
DLL3
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 80 % as determined by SDS-PAGE and Coomassie blue staining.
DLL3
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in PBS, 360 mM NaCl, 200 mM L-arginine ( pH 8.0).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SCDO1 Protein, pu Protein, pudgy Protein, delta like canonical Notch ligand 3 Protein, delta-like 3 (Drosophila) Protein, DLL3 Protein, Dll3 Protein
Hintergrund
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Molekulargewicht
22.30 kDa. Due to glycosylation, the protein migrates to 24-25 kDa based on Tris-Bis PAGE result.