Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 20 mM Tris,150 mM NaCl ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
ECSCR
(Endothelial Cell Surface Expressed Chemotaxis and Apoptosis Regulator (ECSCR))
ARIA Protein, ECSM2 Protein, 1110006O17Rik Protein, endothelial cell surface expressed chemotaxis and apoptosis regulator Protein, ECSCR Protein, Ecscr Protein
Hintergrund
Endothelial cell-specific chemotaxis receptor (ECSCR) is a cell surface protein expressed by blood endothelial cells with roles in endothelial cell migration and signal transduction. Zebrafish ecscr is expressed in angioblasts and in axial vessels during angioblast migration and vasculogenesis. Morpholino-directed ecscr knockdown resulted in defective angioblast migration in the posterior lateral plate mesoderm, a process known to depend on vascular endothelial-derived growth factor (VEGF).
Molekulargewicht
38.6 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.