Dieses EPH Receptor B2 Protein ist gelabelt mit His tag.
Verwendungszweck
Cynomolgus EPHB2 Protein
Sequenz
Val19-Pro542
Produktmerkmale
Recombinant Cynomolgus EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Pro542.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
EPHB2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
Molekulargewicht
59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.