Dieses FAP Protein ist gelabelt mit His-Avi Tag,Biotin.
Sequenz
Leu26-Asp760
Reinheit
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Biotinylated Human FAP, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. See testing image for detail.
FAP
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 20 mM Tris, 150 mM NaCl, 200 mM L-arginine ( pH 8.2).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
FAP, FAPalpha, SIMP, Seprase, APCE, DPPIV, DPPIVA, FAPA,Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Molekulargewicht
87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.