GDNF Protein (AA 78-211)
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- Target Alle GDNF Proteine anzeigen
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 78-211
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- Human GDNF Protein
- Sequenz
- Ser78-Ile211
- Produktmerkmale
- Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
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Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (His tag)
GDNF Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 1-211) protein (GST tag)GDNF Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 78-211) protein (Fc Tag)GDNF Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- Andere Bezeichnung
- GDNF (GDNF Produkte)
- Hintergrund
- Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
- Molekulargewicht
- 15.06 kDa same as Tris-Bis PAGE result.
- Pathways
- RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration
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