Recombinant Human GFRA3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp32-Trp382.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human GFRA3, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
GFRA3
Spezies: Human
Wirt: Baculovirus infected Insect Cells
Recombinant
> 85 % by SDS - PAGE
SDS
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GFRA3 Protein, GFRalpha3 Protein, Y15110 Protein, GDNF family receptor alpha 3 Protein, glial cell line derived neurotrophic factor family receptor alpha 3 Protein, Gfra3 Protein, GFRA3 Protein
Hintergrund
GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
Molekulargewicht
40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.