Dieses Galectin 3 Protein ist gelabelt mit His tag.
Verwendungszweck
Human Galectin 3 Protein
Sequenz
Ala2-Ile250
Produktmerkmale
Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile250.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
LGALS3
Spezies: Cynomolgus
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Konservierungsmittel
Other preservative
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
Molekulargewicht
27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.