Dieses ErbB2/Her2 Protein ist gelabelt mit His-Avi Tag,Biotin.
Sequenz
Thr23-Thr652
Reinheit
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-Her2 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Her2, His Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
ErbB2/Her2
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
CD340, EGFR2, ErbB2, HER2, HER-2, herstatin, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, NGL, p185erbB2, TKR1, ERBB2, HER-2/neu, NEU, ENV, ENVW, ERVWE1, HERV-7q, HERV-W-ENV, HERV7Q, HERVW, HERVWENV,ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.
Molekulargewicht
72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.