HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Protein-Typ
Recombinant
Proteineigenschaft
AA 32-728
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses HGF Protein ist gelabelt mit His tag.
Verwendungszweck
Human HGF Protein
Sequenz
Gln32-Ser728
Produktmerkmale
Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln32-Ser728.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human HGF, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 46.7ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
HGF
(Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))
Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
Molekulargewicht
the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.