Dieses Interferon gamma Protein ist gelabelt mit His-Avi Tag.
Sequenz
Gln24-Gly161
Reinheit
> 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IFN gamma, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 13ng/ml determined by ELISA. See testing image for detail.
IFNG
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AP, AA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Interferon-gamma, Interferon-γ, interferon, gamma, IFG, IFI, IFN gamma,Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.
Molekulargewicht
19.1 kDa. Due to glycosylation, the protein migrates to 20-35 kDa based on Tris-Bis PAGE result.