IL17 Receptor B (IL17RB)
(Interleukin 17 Receptor B (IL17RB))
Protein-Typ
Recombinant
Proteineigenschaft
AA 18-292
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses IL17 Receptor B Protein ist gelabelt mit His tag.
Verwendungszweck
Human IL-17RB Protein
Sequenz
Arg18-Per292
Produktmerkmale
Recombinant Human IL-17RB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Per292.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-17RB, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
IL17RB
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 85 % as determined by reducing SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
IL17 Receptor B (IL17RB)
(Interleukin 17 Receptor B (IL17RB))
Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.
Molekulargewicht
31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.