IL1RAP
(Interleukin 1 Receptor Accessory Protein (IL1RAP))
Protein-Typ
Recombinant
Proteineigenschaft
AA 21-359
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses IL1RAP Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Human IL-1R3/IL-1 RAcP Protein
Sequenz
Ser21-Glu359
Produktmerkmale
Recombinant Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser21-Glu359.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-1R3 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. See testing image for detail.
IL1RAP
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IL1RAP
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
IL1RAP
(Interleukin 1 Receptor Accessory Protein (IL1RAP))
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
Molekulargewicht
42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.