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IL-7 Protein (AA 26-177)

IL7 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Produktnummer ABIN7274963
  • Target Alle IL-7 (IL7) Proteine anzeigen
    IL-7 (IL7) (Interleukin 7 (IL7))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 26-177
    Spezies
    • 19
    • 10
    • 5
    • 1
    • 1
    • 1
    • 1
    Human
    Quelle
    • 17
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Verwendungszweck
    Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
    Sequenz
    Asp26-His177
    Reinheit
    > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    IL-7 (IL7) (Interleukin 7 (IL7))
    Andere Bezeichnung
    IL-7 (IL7 Produkte)
    Synonyme
    IL7 Protein, IL-7 Protein, A630026I06Rik Protein, Il-7 Protein, hlb368 Protein, interleukin 7 Protein, IL7 Protein, Il7 Protein
    Hintergrund
    IL7, Interleukin-7, IL-7, Lymphopoietin-1, PBGF,Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
    Molekulargewicht
    17.5 kDa same as Tris-Bis PAGE result.
    UniProt
    P13232
    Pathways
    JAK-STAT Signalweg
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