IL-7 Protein (AA 26-177)
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- Target Alle IL-7 (IL7) Proteine anzeigen
- IL-7 (IL7) (Interleukin 7 (IL7))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 26-177
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
- Sequenz
- Asp26-His177
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)
IL7 Spezies: Maus Wirt: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 7 (IL7) (AA 26-177) (Active) protein (His tag)IL7 Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Interleukin 7 (IL7) (AA 26-154) protein (His tag)IL7 Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (AA 26-177) protein (His-IF2DI Tag)IL7 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (Active) protein (His tag)IL7 Spezies: Maus Wirt: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 7 (IL7) (AA 26-177) protein (GST tag)IL7 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- IL-7 (IL7) (Interleukin 7 (IL7))
- Andere Bezeichnung
- IL-7 (IL7 Produkte)
- Hintergrund
- IL7, Interleukin-7, IL-7, Lymphopoietin-1, PBGF,Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
- Molekulargewicht
- 17.5 kDa same as Tris-Bis PAGE result.
- UniProt
- P13232
- Pathways
- JAK-STAT Signalweg
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