Dieses IL-17A/F Protein ist gelabelt mit His-Avi Tag.
Verwendungszweck
Mouse IL-17A & F Protein
Sequenz
Ala26-Ala158 (IL-17A) & Arg29-Ala161 (IL-17F)
Spezifität
Uni-Prot: Q62386 (IL-17A), Q7TNI7-1 (IL-17F)
Produktmerkmale
Recombinant Mouse IL-17A & F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Ala158(IL-17A) & Arg29-Ala161(IL-17F).
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse IL-17R alpha, hFc Tag with the EC50 of 19.8ng/ml determined by ELISA. See testing image for detail.
IL-17A/F
Spezies: Human
Wirt: Human Cells
Recombinant
Greater than 95 % as determined by reducing SDS-PAGE.
ELISA, MS, SDS, WB
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
il17-5 Protein, si:ch211-63o20.2 Protein, interleukin 17a/f3 Protein, il17a/f3 Protein
Hintergrund
Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Molekulargewicht
17.9 kDa(IL-17A)&14.9 kDa(IL-17F). Due to glycosylation, the protein migrates to 22-25 kDa&27-30 kDa based on Tris-Bis PAGE result.