Dieses IL4 Receptor Protein ist gelabelt mit His tag.
Verwendungszweck
Human IL-4 R alpha/CD124 Protein
Sequenz
Gly24-His232
Produktmerkmale
Recombinant Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-His232.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-4 R alpha, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 11.76ng/ml determined by ELISA. See testing image for detail.
IL4R
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
Molekulargewicht
24.7 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.