Dieses IL5RA Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human IL-5 R alpha/CD125 Protein
Sequenz
Asp21-Glu335
Produktmerkmale
Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
IL5RA
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
IL5RA
Spezies: Human
Wirt: Wheat germ
Recombinant
AP
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin-5 Receptor alpha (IL-5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
Molekulargewicht
38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.