Dieses IL13 Receptor alpha 1 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human IL-13Ra1 Protein
Sequenz
Ala27-Thr343
Produktmerkmale
Recombinant Biotinylated Human IL-13Ra1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Thr343.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 2.8μg/ml determined by ELISA. See testing image for detail.
IL13RA1
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Molekulargewicht
39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result.