TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
Protein-Typ
Recombinant
Proteineigenschaft
Trimer
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFSF14 Protein ist gelabelt mit His-DYKDDDDK Tag.
Verwendungszweck
Human LIGHT/TNFSF14 Trimer Protein
Sequenz
Ser89-Val240
Produktmerkmale
Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ser89-Val240 Trimer.
Reinheit
> 90 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LIGHT Trimer, His tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
TNFSF14
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFSF14
(Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Molekulargewicht
52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.