LILRB1
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))
Protein-Typ
Recombinant
Proteineigenschaft
AA 24-221
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses LILRB1 Protein ist gelabelt mit His tag.
Verwendungszweck
Human LILRB1/CD85j/ILT2 Domain1 & 2 Protein
Sequenz
Gly24-Gly221
Produktmerkmale
Recombinant Human LILRB1/CD85j/IL-T2 Domain1 & 2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LILRB1 Domain1&2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. The affinity constant of 1.9μM as determined in SPR assay (Biacore T200). See testing image for detail.
LILRB1
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
LILRB1
(Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1))
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens.
Molekulargewicht
23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.