LTBR
(Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))
Protein-Typ
Recombinant
Proteineigenschaft
AA 31-227
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses LTBR Protein ist gelabelt mit Fc Tag.
Verwendungszweck
Human LTBR Protein
Sequenz
Gln31-Met227
Produktmerkmale
Recombinant Human LTBR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln31-Met227.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human LIGHT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA. See testing image for detail.
LTBR
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
LTBR
(Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR))
Components of lymphotoxin beta receptor (LTBR)-associated signaling complexes, including TRAF2, TRAF3, NIK, IKK1, and IKK2 have been shown to participate in the coupling of LTBR to NFkappaB.TRAF3 functions as a negative regulator of LTBR signaling via both canonical and non-canonical NFkappaB pathways by two distinct mechanisms. These effects of TRAF3 depletion did not require LTBR signaling and were consistent with autonomous activation of the non-canonical NFkappaB pathway.
Molekulargewicht
48.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.