Dieses NCR1 Protein ist gelabelt mit His tag,Biotin.
Verwendungszweck
Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling)
Sequenz
Gln22-Asn254
Produktmerkmale
Recombinant Biotinylated Human NKp46/NCR1/CD335 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Asn254.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-Nkp46 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKp46, His Tag with the EC50 of 3.1ng/ml determined by ELISA. See testing image for detail.
NCR1
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
> 90 %
SDS, WB, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
Molekulargewicht
27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.