Dieses Oncostatin M Receptor Protein ist gelabelt mit His tag.
Verwendungszweck
Cynomolgus OSMR Protein
Sequenz
Glu28-Ser737
Produktmerkmale
Recombinant Cynomolgus OSMR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu28-Ser737.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus OSMR, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 14.4ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
OSMRB Protein, PLCA1 Protein, oncostatin M receptor Protein, OSMR Protein, Osmr Protein
Hintergrund
OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.
Molekulargewicht
82.04 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.