TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Protein-Typ
Recombinant
Proteineigenschaft
AA 20-211
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFRSF4 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Mouse OX40/TNFRSF4/CD134 Protein
Sequenz
Val20-Pro211
Produktmerkmale
Recombinant Biotinylated Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse OX40 Ligand, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 4.3μg/ml determined by ELISA. See testing image for detail.
TNFRSF4
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
FACS
TNFRSF4
Spezies: Maus
Wirt: Human Cells
Recombinant
> 95 % as determined by SDS-PAGE
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Molekulargewicht
24.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.