Recombinant Cynomolgus Siglec-10 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr17-Asn552.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus Siglec-10, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 42.0ng/ml determined by ELISA. See testing image for detail.
SIGLEC10
Spezies: Maus
Wirt: Human Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Supplied as 0.22μm filtered solution in 25 mM MES, 150 mM NaCl, 0.5M Arginine ( pH 5.0).
Lagerung
-80 °C
Informationen zur Lagerung
Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Molekulargewicht
59.90 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.