Dieses SIGLEC10 Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human Siglec-10 Protein
Sequenz
Met17-Thr546
Produktmerkmale
Recombinant Biotinylated Human Siglec-10 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-Siglec-10, hFc Tag Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-10, His Tag with the EC50 of 92.9ng/ml determined by ELISA. See testing image for detail.
SIGLEC10
Spezies: Maus
Wirt: Human Cells
Recombinant
>95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM Nacl, 200 mM Arginine ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Molekulargewicht
61.13 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.