Dieses alpha 2 Antiplasmin Protein ist gelabelt mit His tag.
Verwendungszweck
Human SERPINF2/A2AP Protein
Sequenz
Met28-Lys491
Produktmerkmale
Recombinant Human SERPINF2/A2AP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met28-Lys491.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human SERPINF2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.49μg/ml determined by ELISA. See testing image for detail.
SERPINF2
Spezies: Rind (Kuh)
Wirt: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, serpin Protein, AI747498 Protein, Pli Protein, Serpimf2 Protein, serpinf2 Protein, serpinf2l Protein, wu:fd57d08 Protein, Dmrs91 Protein, Pedf Protein, serpin family F member 2 Protein, serpin family F member 1 Protein, serine (or cysteine) peptidase inhibitor, clade F, member 2 Protein, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a Protein, SERPINF2 Protein, Serpinf2 Protein, SERPINF1 Protein, serpinf2a Protein, Serpinf1 Protein
Hintergrund
Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP, SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.
Molekulargewicht
52.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.