SIRP AlphaV2 protein (Fc Tag)

Produktnummer ABIN7275655

Produktdetails

Target: SIRP AlphaV2

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Fc Tag

Sequenz: Glu31-Arg369

Reinheit: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD47 at 1μg/ml (100μl/Well). Dose response curve for Human SIRP alpha V2, hFc Tag with the EC50 of 82.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SIRP AlphaV2

Andere Bezeichnung: SIRP alpha V2

Hintergrund: CD172a, BIT, MFR, MYD1, MYD-1, P84, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, SIRP alpha, SIRPA, Sirp-alpha-1, SIRPalpha2, Sirp-alpha-2, Sirp-alpha-3,Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Molekulargewicht: 63.9 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.