Dieses SIRPG Protein ist gelabelt mit His-Avi Tag,Biotin.
Verwendungszweck
Biotinylated Human SIRP gamma/CD172g Protein
Sequenz
Glu29-Pro360
Produktmerkmale
Recombinant Biotinylated Human SIRP gamma/CD172g Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu29-Pro360.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-SIRP alpha Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human SIRP gamma, His Tag with the EC50 of 35.5ng/ml determined by ELISA. See testing image for detail.
SIRPG
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
SIRPG
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
CD172g Protein, SIRP-B2 Protein, SIRPB2 Protein, SIRPgamma Protein, bA77C3.1 Protein, SIRPG Protein, signal regulatory protein gamma Protein, signal-regulatory protein gamma Protein, SIRPG Protein, LOC716034 Protein, LOC102138723 Protein
Hintergrund
Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.
Molekulargewicht
39.7 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.