Dieses Syndecan 1 Protein ist gelabelt mit His tag.
Verwendungszweck
Human Syndecan-1 Protein
Sequenz
Gln23-Gly254
Produktmerkmale
Recombinant Human Syndecan-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Gly254.
Reinheit
> 95 % as determined by Tris-Bis PAGE
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Syndecan-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA. See testing image for detail.
SDC1
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
Greater than 95 % as determined by SDS-PAGE
WB, ELISA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.
Molekulargewicht
25 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.