TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
Protein-Typ
Recombinant
Proteineigenschaft
AA 22-141
Spezies
Human
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TIGIT Protein ist gelabelt mit His tag.
Verwendungszweck
Human TIGIT Protein
Sequenz
Met22-Pro141
Produktmerkmale
Recombinant Human TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met22-Pro141.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human TIGIT, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 18ng/ml determined by ELISA. The affinity constant of 0.17μM as determined in SPR assay (Biacore T200). See testing image for detail.
TIGIT
Spezies: Cynomolgus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
TIGIT
Spezies: Cynomolgus
Wirt: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TIGIT
(T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))
VSIG9 Protein, VSTM3 Protein, WUCAM Protein, ENSMUSG00000071552 Protein, Vstm3 Protein, RGD1563191 Protein, T-cell immunoreceptor with Ig and ITIM domains Protein, T cell immunoreceptor with Ig and ITIM domains Protein, TIGIT Protein, Tigit Protein
Hintergrund
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Molekulargewicht
14 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.