TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Protein-Typ
Recombinant
Proteineigenschaft
AA 23-257
Spezies
Cynomolgus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFRSF1B Protein ist gelabelt mit His tag.
Verwendungszweck
Cynomolgus TNFR2/CD120b/TNFR1B Protein
Sequenz
Leu23-Asp257
Produktmerkmale
Recombinant Cynomolgus TNFR2/CD120b/TNFR1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.
Reinheit
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus TNFR2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
TNFRSF1B
Spezies: Maus
Wirt: Mammalian Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
TNFRSF1B
Spezies: Human
Wirt: Wheat germ
Recombinant
WB, ELISA, AP, AA
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Lagerung
-20 °C,-80 °C
Informationen zur Lagerung
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Molekulargewicht
26.1 kDa. Due to glycosylation, the protein migrates to 49-60 kDa based on Tris-Bis PAGE result.